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Structural insights into the catalytic activity of bacterial polysaccharide depolymerases.
Bacterial polysaccharide depolymerases are involved in the hydrolysis of bacterial exopolysaccharides. Among them, the family GH16 comprises polysaccharide lyases and glycosyl hydrolases. These enzymes degrade polysaccharide substrates by the cleavage of glycosidic bonds to give oligosaccharide products, which are further degraded to individual sugars. GH16 depolymerases display a broad substrate specificity and can hydrolyze cellulose, pectin, and various extracellular polysaccharides. Moreover, the three-dimensional structures of these enzymes can be determined by X-ray crystallography, whereas the mechanisms of substrate binding and the location of catalytic residues can be examined by site-directed mutagenesis. Here, we summarize the biochemical and structural data on GH16 depolymerases and discuss their catalytic mechanism.Q:
List files with same extension in folder, different levels using PowerShell
I have a folder with many files with same name and extension:
KWS1.07
KWS1.10
KWS1.10.30
I am trying to list all the files with the same extension in the same folder.
A:
You can use a Where-Object call to filter to only return files with a specific extension.
Get-ChildItem | Where-Object Extension -eq '*.exe'
Output:
KWS1.07
KWS1.10
KWS1.10.30
Bioreactor-based manufacturing of extracellular matrix-engineered grafts.
In many tissues, the combination of extracellular matrix (ECM) and cells promotes regeneration. Engineering of these extracellular matrices is usually achieved by adding exogenous ECM components in vitro, where often these reagents are added in batch cultures. However, the production of ECM in vitro is limited by the amount of materials available and the problem of batch cultures. Bioreactors provide controllable, confined environments that allow the production of complex mixtures of cells and growth factors in a single vessel. This chapter describes the fabrication of a synthetic extracellular matrix in a bioreactor. A special microfluidic platform was designed to deliver individual ECM proteins to a cell population, where the ECM proteins are expected to 0b46394aab
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